Embryoid body arrays: Parallel cryosectioning of spheroid/embryoid body samples for medium through-put analysis
نویسندگان
چکیده
منابع مشابه
Controlled-size embryoid body formation in concave microwell arrays.
Embryonic stem (ES) cells hold great potential as a renewable cell source for regenerative medicine and cell-based therapy. Despite the potential of ES cells, conventional stem cell culture methods do not enable the control of the microenvironment. A number of microscale engineering approaches have been recently developed to control the extracellular microenvironment and to direct embryonic ste...
متن کاملCompartmentalized embryoid body culture for induction of spatially patterned differentiation
We developed a compartmentalized culture system of single embryoid bodies (EBs) utilizing a through-hole on a membrane to induce spatially patterned differentiation. An EB derived from mouse pluripotent stem cells was immobilized on the through-hole. By introducing a stem cell maintenance medium and a differentiation medium into upper and lower culture compartments, respectively, a localized di...
متن کاملAnalysis of toxic alkaloids in body samples.
Many plants contain toxic alkaloids which may be dangerous to humans. Despite the large number of poisonous plants, cases of fatal plant poisonings are relatively rare. The frequencies of poisonings and the plants involved are often regionally specific. Plant poisonings can be aggregated into three categories: unintended ingestions, intended ingestions, and poisoning due to abuse of plant mater...
متن کاملPerformance Analysis of Parallel N-Body Codes
-body codes are routinely exploited for simulation studies of physical systems, e.g. in the fields of Computational Astrophysics and Molecular Dynamics. Typically, they require only a moderate amount of run-time memory, but are very demanding in computational power. A detailed analysis of an -body code performance, in terms of the relative weight of each task of the code, and how such weight is...
متن کاملA standardized human embryoid body platform for the detection and analysis of teratogens
Teratogens are compounds that can induce birth defects upon exposure of the developing fetus. To date, most teratogen studies utilize pregnant rodents to determine compound teratogenicity in vivo. However, this is a low throughput approach that cannot easily meet the need for comprehensive high-volume teratogen assessment, a goal of the US Environmental Protection Agency. In addition, rodent an...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Stem Cell Research
سال: 2018
ISSN: 1873-5061
DOI: 10.1016/j.scr.2018.02.003